In an effort to enhance soldier lethality, Army researchers are developing biorecognition receptors capable of consistent performance in multi-domain environments with the ability to collect real-time assessments of soldier health and performance.
ARL scientists, in collaboration with researchers from the California Institute of Technology and Indi Molecular, Inc., have developed a protein catalyzed capture (PCC) agent technology that improves previous versions of receptors and could enable the monitoring of personal and environmental data from soldiers in the field.
"PCC technology has demonstrated improvements in receptor stability, adaptability and manufacturability over standard antibody receptors, and supports the Soldier Lethality Cross-Functional Team as a potentially viable technology to monitor soldier performance via relevant biomarkers collectable from wearable sensors," said Dr. Matt Coppock, chemist and team lead for the U.S. Army Combat Capabilities Development Command's Army Research Laboratory. Biological receptors are integrated into a biosensor to selectively capture a target of interest from a complex mixture like blood, sweat, salvia, etc., to produce the measurable effect by the sensor. "Without the receptor, it would be impossible to know you are detecting what you want to detect," Coppock said.
Antibodies collected from animals injected with the target of interest are used as receptors in biological sensors due to their high binding strengths and selectivity for the target.
"The gold standard receptor work is based around antibodies, which are fantastic at target capture and selectivity, but their detection capabilities are somewhat limited due to their instability, limited shelf life and batch-to-batch performance variation," Coppock said. So the research team developed a different and more innovative approach.
"As an alternative, peptide-based receptors are smaller, simpler to produce, inexpensive and much more robust to environmental stresses, while still retaining the desirable binding properties of an antibody," Coppock said.
Receptors used by the research team are capable of retaining nearly all activity after being heated for one hour at 90 degrees Celsius, whereas many antibodies are completely inactive within minutes after heating up to greater than 70 degrees Celsius.
"We utilize an entirely synthetic approach to receptor development, which allows for much more control over the incorporation of unique building blocks to guarantee stability and permit straightforward modifications for sensor integration," Coppock said.
The team built a full infrastructure of capabilities that allows them to fully develop the synthetic, peptide-based receptors in house at the laboratory, on-demand and in whatever quantities are needed through widely available peptide synthesizers. Researchers are now capable of fully developing a receptor from start to finish in as little as two to three weeks once the target of interest is identified. This process previously took about five to six months.
The team noted that research continues on making the design and selection process of new reagents in a more rapid fashion to make these sensors easier to manufacture on demand. In addition, ARL is currently coordinating efforts to address sensing needs in human performance and food and water safety.